# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 24 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 24.0 896.8 26.2 0.2 20.9 0.1 24.1 894.6 27.7 0.3 22.4 0.1 24.2 860.7 29.6 0.3 24.3 0.2 24.3 721.6 32.3 0.0 27.3 0.0 24.4 636.2 35.8 0.0 30.5 0.0 24.5 563.9 39.3 0.1 34.3 0.0 24.6 523.4 41.7 0.0 37.3 0.0 24.7 286.9 57.3 1.4 51.3 0.5 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 25 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 25.0 893.5 26.2 0.5 21.1 0.3 25.1 832.2 27.6 0.0 22.8 0.1 25.2 815.8 29.3 0.0 24.4 0.1 25.3 730.4 32.2 0.1 27.3 0.3 25.4 646.1 34.7 0.0 29.9 0.1 25.5 622.0 37.5 0.0 33.0 0.0 25.6 572.7 40.4 0.0 35.9 0.0 25.7 443.5 45.6 0.1 40.2 0.1 25.8 387.6 49.8 0.2 44.5 0.2 25.9 321.9 55.0 0.2 49.3 0.1 26.1 283.6 58.8 0.1 53.3 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 26 07102014 hydroquinone cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 26.0 998.6 24.9 0.1 19.7 0.3 26.1 919.8 26.8 0.1 21.7 0.1 26.2 866.1 28.8 0.0 23.7 0.1 26.3 866.1 30.3 0.1 25.3 0.1 26.4 791.7 32.0 0.2 26.9 0.2 26.5 740.2 34.1 0.0 29.3 0.2 26.6 743.5 33.2 0.1 28.0 0.2 26.7 681.1 36.5 0.1 31.3 0.0 26.8 582.5 42.2 0.0 37.0 0.0 26.9 555.2 43.1 0.3 37.9 0.1